Differences in metabolite levels upon differentiation of intact neuroblastoma × glioma cells observed by proton NMR spectroscopy

FEBS Letters

Cohen, Jack S.²  Burrows, Howard¹  Navon, Gil² 

[1] Laboratory of Biochemical Genetics, National Heart Lung and Blood Institute, National Institutes of Health, Bethesda, MD 20205, USA;Laboratory of Theoretical and Physical Biology, National Institute of Child Health and Human Development, Bethesda, MD 20205, USA

关键词: Differentiation;    Neuroblastoma × glioma cell;    Proton NMR vs spin-echo;    Glutamine;    Theophylline;    Prostaglandin E1;   

DOI  :  10.1016/0014-5793(83)80780-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proton NMR spectroscopy was used to study the effect of differentiation with prostaglandin E₁ and theophylline on intact hybrid neuroblastoma × glioma cells. The standard proton NMR method showed more resolvable signals than the spin echo NMR spectra. The differentiated cells were found to contain significantly higher levels of glutamine than the undifferentiated precursors. Observations on cell extracts confirmed these results.

【 授权许可】

Unknown