Local administration of prostaglandin E1 combined with silicone chamber improves peripheral nerve regeneration

INTERNATIONAL JOURNAL OF SURGERY

卷:11

Article

Najafpour, Alireza¹  Mohammadi, Rahim²  Faraji, Darab¹  Amini, Keyvan³ 

[1] Islamic Azad Univ, Urmia Branch, Fac Vet Med, Dept Clin Sci, Orumiyeh, Iran

[2] Urmia Univ, Dept Clin Sci, Fac Vet Med, Orumiyeh 571531177, Iran

[3] Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N 5B4, Canada

关键词: Peripheral nerve repair;    Sciatic;    Prostaglandin E1;    Local;   

DOI  :  10.1016/j.ijsu.2013.05.034

来源: Elsevier

【 摘 要 】

The aim of this study was to assess the effect of locally administered prostaglandin E1 on peripheral nerve regeneration and functional recovery. Sixty male healthy white Wistar rats were divided into four experimental groups (n = 15), randomly: In transected group (TC), left sciatic nerve was transected and stumps were fixed in the adjacent muscle. In treatment group defect was bridged using silicone graft (SIL/PE) filled with 10 mu L prostaglandin E1. In silicone graft group (SIL), the graft was filled with phosphate-buffered saline alone. In sham-operated group (SHAM), sciatic nerve was exposed and manipulated. Each group was subdivided into three subgroups of five animals each and regenerated nerve fibers were studied 4, 8 and 12 weeks after surgery. Behavioral testing, sciatic nerve functional study, gastrocnemius muscle mass and morphometric indices confirmed faster recovery of regenerated axons in SIL/PE than SIL group (p < 0.05). In immunohistochemistry, location of reactions to S-100 in SIL/PE was clearly more positive than that in SIL group. When loaded in a silicone graft, prostaglandin E1 improved functional recovery and morphometric indices of sciatic nerve. Local application of prostaglandin E1 improved functional recovery and morphometric indices of sciatic nerve. (C) 2013 Surgical Associates Ltd. Published by Elsevier Ltd. All rights reserved.

【 授权许可】

Free