Effect of heparin on proliferation and signalling in BC3H‐1 muscle cells

FEBS Letters

Vannucchi, Simonetta²  Biabchini, Pietro³  Pasquali, Franca²  Ruggiero, Marco²  Fiorelli, Gianna¹ 

[1] Dipartimento di Fisiopatologia Clinica, University of Firenze, Viale Morgagni 50, 50134 Firenze, Italy;Istituto di Patologia Generale, Firenze, Italy;Opocrin Research Laboratories, Modena, Italy

关键词: Heparin;    Cell growth;    Inositol lipid;    Cell signalling;    EGF;    epidermal growth factor;    PDGF;    plateletderived growth factor;    IGF-1;    insulin-like growth factor-1;    ECGF;    endothelial cell growth factor;    a-FGF;    acidic fibroblast growth factor;    GAGs;    glycosaminoglycans;    VLMW-heparin;    very low molecular weight heparin;    BSA;    bovine serum albumin;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(90)80723-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied binding and growth inhibitory properties of different glycosaminoglycans in growing and differentiated BC3H-1 muscle cells. Heparin (10 ) and heparan sulfate (10 ) significantly inhibited DNA synthesis in growing and differentiated cells, as monitored by [³H] thymidine incorporation. Binding of heparin to BC3H-1 cells was specific and time-dependent. Heparan sulfate was the only glycosaminoglycan able to displace [³H]heparin (IC₅₀, 3.2 × 10⁻⁷ M), although it was 10-fold less effective than heparin itself (IC⁵⁰ 3.6 × 1O⁻⁸ M). Scatchard analysis revealed the existence of high-affinity hepariy binding sites (K_d, 5 × 10⁻⁸ M). Furthermore, heparin inhibited serum-induced stimulation of inositol lipid turnover. Taken together, these results indicate that heparin inhibits BC3H-1 cell growth by interacting with the cell surface, possibly disrupting the flow of growth factor-related mitogenic signalling.

【 授权许可】

Unknown