FEBS Letters | |
Effect of heparin on proliferation and signalling in BC3H‐1 muscle cells | |
Vannucchi, Simonetta2  Biabchini, Pietro3  Pasquali, Franca2  Ruggiero, Marco2  Fiorelli, Gianna1  | |
[1] Dipartimento di Fisiopatologia Clinica, University of Firenze, Viale Morgagni 50, 50134 Firenze, Italy;Istituto di Patologia Generale, Firenze, Italy;Opocrin Research Laboratories, Modena, Italy | |
关键词: Heparin; Cell growth; Inositol lipid; Cell signalling; EGF; epidermal growth factor; PDGF; plateletderived growth factor; IGF-1; insulin-like growth factor-1; ECGF; endothelial cell growth factor; a-FGF; acidic fibroblast growth factor; GAGs; glycosaminoglycans; VLMW-heparin; very low molecular weight heparin; BSA; bovine serum albumin; PBS; phosphate-buffered saline; | |
DOI : 10.1016/0014-5793(90)80723-V | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We studied binding and growth inhibitory properties of different glycosaminoglycans in growing and differentiated BC3H-1 muscle cells. Heparin (10 ) and heparan sulfate (10 ) significantly inhibited DNA synthesis in growing and differentiated cells, as monitored by [3H] thymidine incorporation. Binding of heparin to BC3H-1 cells was specific and time-dependent. Heparan sulfate was the only glycosaminoglycan able to displace [3H]heparin (IC50, 3.2 × 10−7 M), although it was 10-fold less effective than heparin itself (IC50 3.6 × 1O−8 M). Scatchard analysis revealed the existence of high-affinity hepariy binding sites (Kd, 5 × 10−8 M). Furthermore, heparin inhibited serum-induced stimulation of inositol lipid turnover. Taken together, these results indicate that heparin inhibits BC3H-1 cell growth by interacting with the cell surface, possibly disrupting the flow of growth factor-related mitogenic signalling.
【 授权许可】
Unknown
【 预 览 】
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