Distinct phospholipase C‐regulated signalling pathways in Swiss 3T3 fibroblasts induce the rapid generation of the same polyunsaturated diacylglycerols

FEBS Letters

Pettitt, Trevor R¹  Wakelam, Michael J.O¹ 

[1] Institute for Cancer Studies, University of Birmingham Medical School, Birmingham, B15 2TA, UK

关键词: Cell signalling;    Diacylglycerol;    Phospholipase C;    Protein kinase C;    Swiss 3T3;    DRG;    diradylglycerol;    DAG;    diacylglycerol;    PtdOH;    phosphatidic acid;    PtdCho;    phosphatidylcholine;    PtdEtn;    phosphatidylethanolamine;    PtdIns;    phosphatidylinositol;    PtdInsP2;    phosphatidylinositol 4;    5-bisphosphate;    PtdBut;    phosphatidylbutanol;    PtdSer;    phosphatidylserine;    IP3;    inositol 1;    4;    5-trisphosphate;    EGF;    epidermal growth factor;    PDGF;    platelet-derived growth factor;    PGF2α;    prostaglandin F2α;    TPA;    12-O-tetradecanoyl phorbol 13-acetate;    PLC;    phospholipase C;    PLD;    phospholipase D;    PAP;    phosphatidate phosphohydrolase;    PKC;    protein kinase C;    DNB;    3;    5-dinitrobenzoyl (derivative);    HBS;    Hanks' buffered saline;   

DOI  :  10.1016/S0014-5793(98)00471-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prostaglandin F_2α, platelet-derived growth factor (PDGF) and calcium ionophore A23187 stimulated the rapid (within 25 s) generation of polyunsaturated 1,2-diacylglycerol (DAG) species, in particular 18:0/20:3n-9, 18:0/20:4n-6 and 18:0/20:5n-3, in Swiss 3T3 fibroblasts. This was followed by a second sustained phase characterised by saturated, monounsaturated and diunsaturated DAG species derived, at least partially, from a phospholipase D/phosphatidate phosphohydrolase-linked pathway. This could be directly activated by phorbol ester. Assay of rat brain protein kinase C (PKC) in lipid vesicles showed that first phase, polyunsaturated-enriched DAG isolated from Swiss 3T3 cells was a more potent activator of kinase activity compared to that achieved with DAG from control or 5 min stimulated cells. Thus activation of distinct members of the phospholipase C family leads to the rapid and almost identical generation of polyunsaturated DAG species which are capable of preferentially activating protein kinase C (PKC).

【 授权许可】

Unknown