FEBS Letters | |
Effect of D609 on phosphatidylcholine metabolism in the nuclei of LA‐N‐1 neuroblastoma cells: a key role for diacylglycerol | |
Antony, Pierre1  Horrocks, Lloyd A.2  Farooqui, Akhlaq A.2  Freysz, Louis1  | |
[1] Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Institut de Chimie Biologique, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France;Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210-1218, USA | |
关键词: Phospholipase C; Diacylglycerol; D609 (tricyclodecan-9-yl xanthogenate); LA-N-1 nuclei; CTP:phosphocholine cytidylyltransferase; Phosphatidylcholine cycle; PLC; phospholipase C; PLD; phospholipase D; PtdCho; phosphatidylcholine; PtdEtOH; phosphatidylethanol; PtdIns; phosphatidylinositol; CCT; CTP:phosphocholine cytidylyltransferase; DAG; diacylglycerol; TPA; 12-O-tetradecanoylphorbol-13-acetate; | |
DOI : 10.1016/S0014-5793(01)03149-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In our previous studies, TPA treatment of LA-N-1 cells stimulated the production of diacylglycerol in nuclei, probably through the activation of a phospholipase C. Stimulation of the synthesis of nuclear phosphatidylcholine by the activation of CTP:phosphocholine cytidylyltransferase was also observed. The present data show that both effects were inhibited by the pretreatment of the cells with D609, a selective phosphatidylcholine-phospholipase C inhibitor, indicating that the diacylglycerol produced through the hydrolysis of phosphatidylcholine in the nuclei is reutilized for the synthesis of nuclear phosphatidylcholine and is required for the activation of CTP:phosphocholine cytidylyltransferase.
【 授权许可】
Unknown
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