Effect of D609 on phosphatidylcholine metabolism in the nuclei of LA‐N‐1 neuroblastoma cells: a key role for diacylglycerol

FEBS Letters

Antony, Pierre¹  Horrocks, Lloyd A.²  Farooqui, Akhlaq A.²  Freysz, Louis¹ 

[1] Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Institut de Chimie Biologique, Faculté de Médecine, 11 rue Humann, 67085 Strasbourg Cedex, France;Department of Molecular and Cellular Biochemistry, The Ohio State University, Columbus, OH 43210-1218, USA

关键词: Phospholipase C;    Diacylglycerol;    D609 (tricyclodecan-9-yl xanthogenate);    LA-N-1 nuclei;    CTP:phosphocholine cytidylyltransferase;    Phosphatidylcholine cycle;    PLC;    phospholipase C;    PLD;    phospholipase D;    PtdCho;    phosphatidylcholine;    PtdEtOH;    phosphatidylethanol;    PtdIns;    phosphatidylinositol;    CCT;    CTP:phosphocholine cytidylyltransferase;    DAG;    diacylglycerol;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/S0014-5793(01)03149-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In our previous studies, TPA treatment of LA-N-1 cells stimulated the production of diacylglycerol in nuclei, probably through the activation of a phospholipase C. Stimulation of the synthesis of nuclear phosphatidylcholine by the activation of CTP:phosphocholine cytidylyltransferase was also observed. The present data show that both effects were inhibited by the pretreatment of the cells with D609, a selective phosphatidylcholine-phospholipase C inhibitor, indicating that the diacylglycerol produced through the hydrolysis of phosphatidylcholine in the nuclei is reutilized for the synthesis of nuclear phosphatidylcholine and is required for the activation of CTP:phosphocholine cytidylyltransferase.

【 授权许可】

Unknown