Heparin inhibits A431 cell growth independently of serum and EGF mitogenic signalling

FEBS Letters

Vannucchi, Simonetta¹  Pasquali, Franca¹  Ruggiero, Marco¹  Chiarugi, Vincenzo P.¹ 

[1] Istituto di Patologia Generale, University of Florence, Viale Morgagni 50, 50134 Florence, Italy

关键词: Heparin;    Epidermal growth factor;    Inositol lipid;    A431 carcinoma cells;    Cell growth;    EGF;    Epidermal growth factor;    APTT;    activated partial thromboplastin time;    AXa;    anti-activated coagulation Factor X activity;    TCA;    trichloroacetic acid;    DMEM;    Dulbecco's modified Eagle medium;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(91)80378-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In several cell types heparin exerts an antiproliferative action; here we report that heparin inhibited the growth of human epidermoid carcinoma A431 cells. Heparin binding to the cell surface was necessary for growth inhibition; binding was influenced by the molecular weight of heparin. Inhibition of A431 cell proliferation was evident in the presence and in the absence of serum, thus indicating that heparin did not act by binding and ‘subtracting’ nutrients or other serum factors. In confluent A431 cells, EGF induced DNA synthesis, but heparin did not inhibit this effect; consistently, it did not affect inositol lipid turnover triggered by EGF or bradykinin.

【 授权许可】

Unknown