FEBS Letters | |
Transcription and replication silencer element is present within conserved region of human Alu repeats interacting with nuclear protein | |
Iguchi-Ariga, Sanae M.M.2  Ariga, Hiroyoshi2  Tomilin, Nikolai V.1  | |
[1] Institute of Cytology, The Academy of Sciences of the USSR, 194064 Leningrad, USSR;Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan | |
关键词: Alu sequence element; Chloramphenicolacetyltransferase assay; Silencer; | |
DOI : 10.1016/0014-5793(90)80707-P | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Human cells contain a nuclear protein interacting with Alu repeats, and this protein seems to recognize a conserved sequence motif, GGAGGC, present within the RNA polymerase III promoter and within the SV40 T-antigen-dependent ARS-like element. To study the potential functional role of this element, we have inserted the sequence into a chloramphenicolacetyltransferase (CAT) expression vector with a SV40 promoter and enhancer element from the up-stream region of the human c-myc gene, and transfected HeLa cells with the resulting plasmid. Analysis of expression by the CAT assay indicates that the Alu-derived sequence supresses transcription of the CAT gene driven by the c-myc enhancer/SV40 promoter. The Alu-derived sequence also inhibits ARS activity of the c-myc enhancer. The data allow the explaination of the transcriptional inactivity of Alu repeats in HeLa cells, and suggest the existence of a negative control of Alu transcription.
【 授权许可】
Unknown
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