Contribution of a PS1‐like element to the tissue‐ and cell‐specific expression of the human GLP‐1 receptor gene

FEBS Letters

Galehshahi, Firouzeh Shoghi¹  Lankat-Buttgereit, Brigitte¹  Göke, Burkhard² 

[1] Clinical Research Unit for Gastrointestinal Endocrinology, Philipps-University of Marburg, Baldingerstr., D-35033 Marburg, Germany;Department of Gastroenterology, Inselspital, University of Bern, CH-3010 Bern, Switzerland

关键词: GLP-1 receptor promoter;    cis-regulatory element;    PS1;    Silencer;   

DOI  :  10.1016/S0014-5793(98)01116-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The GLP-1 receptor (GLP-1R) mediates the insulinotropic effects of the incretion hormone glucagon-like peptide 1 (7–36) amide (GLP-1). Recently, we cloned the 5′-flanking region of the human GLP-1R gene. To characterize tissue- and cell-specific cis-regulatory elements, we constructed a series of 5′-deletions of the promoter. The activity of these constructs was tested in different cell lines. An element with high homology to PS1 was found to repress GLP-1R promoter activity in fibroblasts and pancreatic D-cells, but was not active in pancreatic A- and B-cells. PS1 was described to inhibit activation of a D-cell-specific enhancer. Cloning the PS1-like element upstream a heterologous promoter (SV40) revealed that it is functionally active independently from this enhancer. Our data suggest that basal activity of the GLP-1R promoter is silenced in a tissue- and cell-specific manner by negatively acting cis-regulatory elements, including a PS1-like element.

【 授权许可】

Unknown