Regulation of human renin secretion and gene transcription in Calu‐6 cells

FEBS Letters

Pinet, Florence¹  Corvol, Pierre¹  Fuchs, Sébastien¹  Lengronne, Armelle¹  Philippe, Josette¹  Germain, Stéphane¹ 

[1] INSERM Unit 36, Collège de France, 3 rue d'Ulm, 75005 Paris, France

关键词: Renin;    cAMP;    Promoter;    Intron;    Silencer;    Serum;    bp;    base pair;    CAT;    chloramphenicol acetyl transferase;    CRE;    cAMP-responsive element;    CREB;    CRE-binding protein;    FCS;    fetal calf serum;    JG;    juxtaglomerular;   

DOI  :  10.1016/S0014-5793(97)00332-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calu-6 cells were characterized for studying the transcriptional regulation of the human renin gene. Analysis of cis-acting elements of the renin promoter showed the highest activity within the first 582 bp in serum-free conditions and of the 892 bp in the presence of serum. cAMP activates renin mRNA synthesis parallel to renin production (20-fold increase) as well renin promoter activity (2-fold). cAMP response element and the (−77 to −67) element are both necessary for activation of the renin promoter but do not act independently. Functional analysis of Intron A revealed the presence of a silencer specific to renin-producing cells.

【 授权许可】

Unknown