An intronic promoter controls the expression of truncated human γ‐glutamyltransferase mRNAs

FEBS Letters

Leh, Hervé²  Guellaën, Georges¹  Visvikis, Athanase²  Wellman, Maria²  Ichino, Kazuhiko²  Siest, Gérard²  Chikhi, Naı̈ma¹ 

[1] Unité INSERM U99, Hôpital Henri Mondor, 94010 Créteil, France;Centre du Médicament, Faculté de Pharmacie UHP Nancy 1, 30 rue Lionnois, 54000 Nancy, France

关键词: γ-Glutamyltranspeptidase;    Truncated mRNA;    Intron;    Promoter;    GGT;    γ-glutamyltransferase;    kb;    kilobase;    UTR;    untranslated region;    Denhardt's;    1% Ficoll;    1% polyvinylpyrolidone;    and 1% bovine serum albumin;    PCR;    polymerase chain reaction;    1×SSC;    0.15 M NaCl/0.015 M sodium citrate;   

DOI  :  10.1016/S0014-5793(98)00950-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified and characterized a genomic DNA fragment containing the coding sequences corresponding to the human γ-glutamyltransferase type 1 mRNA. The coding part of the gene spans over 16 kb and comprises 12 exons and 11 introns exhibiting a similar organization as for the mouse and rat GGT genes. The exons 1–7 encode the heavy subunit whereas exons 8–12 which encode the carboxy-terminal part of the heavy subunit (exon 8) and the light subunit are clustered in a 1.6-kb BglII fragment. Exons 7 and 8 are separated by a 3.9-kb intron containing in its 3′ part the sequences corresponding to the 5′-UTRs of the truncated GGT mRNAs described for human lung. Sequence analysis upstream this transcribed region exhibited putative promoter sequences and after transient transfection significant promoter activities were measured in V79 lung fibroblasts and KYN-2 hepatoma cells but not in A2780 ovarian cells. This specificity disappeared when only 550 bp upstream the transcription start site were used as promoter. These results argue for a promoter of truncated GGT mRNAs in intron 7, specifically regulated in human tissues.

【 授权许可】

Unknown