Cloning and expression of a novel type (III) of human γ‐glutamyltransferase truncated mRNA

FEBS Letters

Leh, Hervé¹  Gerardin, Philippe¹  Visvikis, Athanase¹  Wellman, Maria¹  Courtay, Céline¹  Siest, Gérard¹ 

[1] Centre du Médicament (Université Henri Poincaré (Nancy 1)), URA CNRS 597, 30 Rue Lionnois, 54000 Nancy, France

关键词: γ-Glutamyltranspeptidase;    Multigenic family;    Truncated mRNA;    Leukemia;    ALL;    acute lymphoblastic leukemia;    GGT;    γ-glutamyltransferase;    kb;    kilobases;    ORF;    open reading frame;    RT;    reverse transcriptase;    1×SSC;    0.15 M NaCl/0.015 M sodium citrate;   

DOI  :  10.1016/0014-5793(96)00965-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the characterization of a novel human γ-glutamyltransferase mRNA type. This type III mRNA differs from type I and type II mRNAs previously described by several point mutations and the presence of an unspliced 81 bp intron in the open reading frame. Further, type III mRNAs are truncated ones and are tissue and pathology specifically expressed. In fact, type III mRNAs are present in human placenta, sigmoid, lung and in 50% of acute lymphoblastic leukemia blood cells but they are never found in healthy lymphocytes.

【 授权许可】

Unknown