Protein kinase C during differentiation of human promyelocytic leukemia cell line, HL‐60

FEBS Letters

Yasuda, Ichiro¹  Kishimoto, Akira¹  Aihara, Hiroaki¹  Hashimoto, Keisuke¹  Nishizuka, Yasutomi¹  Mikawa, Katsuya¹ 

[1] Department of Biochemistry, Kobe University School of Medicine, Kobe 650, Japan

关键词: Protein kinase C;    Retinoic acid;    Differentiation;    (HL-60 cells);    PKC;    protein kinase C;    RA;    retinoic acid;    DMSO;    dimethyl sulfoxide;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    PS;    phosphatidylserine;    DO diolein;    EGTA [ethylenebis(oxyethylenenitrilo)]tetraacetic acid;   

DOI  :  10.1016/0014-5793(90)80698-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C (PKC) from human promyelocytic leukemia HL-60 cells can be resolved into three fractions (peak a, b and c) by hydroxyapatite column chromatography. Peak a and c enzymes are indistinguishable from the brain type II PKC having β (βI and βII)-sequence and type III having α-sequence, respectively. Peak b enzyme is a previously unidentified PKC subspecies that has enzymological properties subtly different from type I (having γ-sequence), type II and type III PKC. Upon treatment of HL-60 cells with 1 μM retinoic add, this peak b enzyme is decreased dramatically within 24 h, whilst peak a enzyme (β-PKC) is increased, and peak c (α-PKC) enzyme is slightly decreased within 48 h. The result implies that the PKC subspecies in HL-60 cells have distinct functions during cell differentiation.

【 授权许可】

Unknown