FEBS Letters | |
Retinoic acid inhibits phospholipid turnover and protein kinase C activity in RA‐sensitive but not in RA‐resistant cells | |
Banerjee, D.1  Mukherjee, B.B.1  Vanier, M.C.1  | |
[1] Department of Biology and Centre for Human Genetics, McGill University, 1205 Dr Penfield Ave., Montreal, Quebec H3A 1B1, Canada | |
关键词: Retinoic acid; Phospholipid turnover; Protein kinase C; Anchorage-independent growth; RA; all-trans-retinoic acid; PKC; protein kinase C; PC; phosphatidylcholine; PS; phosphatidylserine; PI; phosphatidylinositol; PE; phosphatidylethanolamine; PA; phosphatidic acid; | |
DOI : 10.1016/0014-5793(88)81050-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Treatment with 10−5 M retinoic acid causes loss of anchorage-independent growth in src-transformed RR1022 cells but not in ras-transformed KNRK cells. In an effort to elucidate the mechanisms underlying this difference, we investigated the effect of RA on phospholipid turnover and PKC activity in these two cell lines. 10−5 M RA treatment caused a drastic inhibition of 32P incorporation into PI and PA and a large increase in 32P incorporation into PC in RR1022 cells. Similar treatment of KNRK cells yielded no change in PC or PA labelling and a much smaller decrease in PI labelling. Furthermore, 10−5 M RA treatment causes a large decrease in PKC activity in RR1022 cells (35% of control) but only a small decrease in KNRK cells (78% of control). We suggest that these effects are part of an altered signal transduction pathway which mediates the differential effects of RA on anchorage-independent growth in these two cell lines.
【 授权许可】
Unknown
【 预 览 】
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