Activation of c‐Jun amino‐terminal kinase is required for retinoic acid‐induced neural differentiation of P19 embryonal carcinoma cells

FEBS Letters

Guang, Li Ming¹  Kanno, Shin-ichiro²  Tamura, Shinri¹  Wang, Hong¹  Kobayashi, Takayasu¹  Sasaki, Masato¹  Ohnishi, Motoko¹  Ikeda, Shoko¹ 

[1] Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University, 4-1 Seiryomachi, Aoba-ku, Sendai 980-8575, Japan;Department of Molecular Genetics, Institute of Development, Aging and Cancer, Tohoku University, Sendai 980-8575, Japan

关键词: P19 embryonal carcinoma cell;    Retinoic acid;    Neural differentiation;    c-Jun amino-terminal kinase;    Okadaic acid-sensitive protein phosphatase;    Protein kinase C;    RA;    retinoic acid;    JNK;    c-Jun amino-terminal kinase;    PKC;    protein kinase C;    NF-L;    neurofilament-L;    PCR;    polymerase chain reaction;    OA;    okadaic acid;   

DOI  :  10.1016/S0014-5793(01)02699-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

P19 embryonal carcinoma cells are known to differentiate into neurons and glia when treated with relatively high concentrations (>100 nM) of retinoic acid (RA). Concomitant with this RA-induced neural differentiation, we observed an activation of the c-Jun amino-terminal kinase (JNK). JNK was required for the RA-induced neural differentiation, because dominant-negative JNK blocked the differentiation. Studies using protein phosphatase inhibitors and protein kinase inhibitors suggested that both okadaic acid-sensitive protein phosphatase(s) and protein kinase C participate in the RA-induced activation of JNK.

【 授权许可】

Unknown