Cellular HSP90 (HSP86) mRNA level and in vitro differentiation of mouse embryonal carcinoma (F9) cells

FEBS Letters

Oishi, Michio¹  Kohda, Takashi¹  Kondo, Kazuhiro¹ 

[1] Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Differentiation;    Embryonal carcinoma;    Heat-shock protein;    HSP90;    Retinoic acid;    Cyclic AMP;   

DOI  :  10.1016/0014-5793(91)81237-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of mouse embryonal carcinoma (F9) cells with retinoic acid, an inducer of F9 cell differentiation, greatly increased the level of mRNA specific to one of the heat-shock proteins (HSP86). Experiments including the one employing differentiation-resistant mutant F9 cells suggested that the increase represents early molecular events associated with the embryonal differentiation. The increased HSP86 mRNA declined to the original level during further incubation. The presence of cyclic AMP, which stimulates conversion of the retinoic acid-induced primitive endoderm cells to parietal endoderm cells, prevented the decline. These results suggest that not only the elevation of HSP86 mRNA level represents early molecular events in F9 cell differentiation but also that sustaining the elevated level (by cyclic AMP) is associated with further differentiation of the embryonal cells.

【 授权许可】

Unknown