Different expression time of the 105‐kDa protein and 90‐kDa heat‐shock protein in rat testis

FEBS Letters

Tashima, Yohtalou¹  Itoh, Hideaki¹ 

[1] Department of Biochemistry, Akita University School of Medicine, Japan

关键词: Heat-shock protein;    Stress protein;    HSP90;    105-kDa Protein;    HSP90 and HSP100;    90-kDa and 100-kDa heat-shock protein;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    PVDF;    polyvinylidene difluoride;    MEM;    Eagle's minimum essential medium;   

DOI  :  10.1016/0014-5793(91)80920-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To understand the physiological functions of the 105-kDa protein which is testis-specific and HSP90 (90-kDa heat-shock protein) related protein. the appearance of it in the testis has been followed during the development of rat. On immunoblotting analysis. the 105-kDa protein did not appear until after the age of five weeks, while HSP90 could be detected at three weeks. In the spermatozoa. the 105-kDa protein was much abundant but not in the LC-540 cells (a cell line from Leydig cell tumor in rat testis) cytosol. This finding has attracted much attention to the relationship between this protein and sperm functions.

【 授权许可】

Unknown