Nitric oxide is involved in heat‐induced HSP70 accumulation

FEBS Letters

Mikoyan, V.D.²  Manukhina, E.B.¹  Kubrina, L.N.²  Malyshev, I.Yu.¹  Vanin, A.F.² 

[1] Institute of General Pathology and Pathophysiology, Baltijskaya 8, Moscow 125315, Russian Federation;Institute of Chemical Physics, Kosygin Str. 4, Moscow 117977, Russian Federation

关键词: Heat shock;    EPR;    Western blot analysis;    Nitric oxide;    Stress protein;    BP;    blood pressure;    DETC;    Fe2+-diethyldithiocar-bamate;    EPR;    electron paramagnetic resonance;    HS;    heat shock;    HSP70 heat shock proteins with molecular weight 70 kDa;    L-NNA;    Nω-nitro-L-arginine;    MNIC;    mononitrosyl-iron complex;    NO;    nitric oxide;    cNOS;    constituitive NO-synthase;    iNOS;    inducible NO-synthase;   

DOI  :  10.1016/0014-5793(95)00801-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heat shock potentiated the nitric oxide production (EPR assay) in the liver, kidney, heart, spleen, intestine, and brain. The heat shock-induced sharp transient increase in the rate of nitric oxide production preceded the accumulation of heat shock proteins (HSP70) (Western blot analysis) as measured in the heart and liver. In all organs the nitric oxide formation was completely blocked by the NO-synthase inhibitor N^ω-nitro-L-arginine (L-NNA). L-NNA also markedly attenuated the heat shock-induced accumulation of HSP70. The results suggests that nitric oxide is involved in the heat shock-induced activation of HSP70 synthesis.

【 授权许可】

Unknown