Undifferentiated HL60 cells respond to extracellular ATP and UTP by stimulating phospholipase C activation and exocytosis

FEBS Letters

Stutchfield, Jane¹  Cockcroft, Shamshad¹ 

[1]Department of Physiology, Rockefeller Building, University College London, University Street, London WC1E6JJ, UK

关键词: ATP receptor;    UTP receptor;    Inositol phosphate;    Secretion;    Cytosol Ca2+;    Differentiation;    ATPγS;    adenosine 5'-[γ-thio]triphosphate;    GTPγS;    guanosine 5'-[γ-thio]triphosphate;    AppNHp;    adenosine 5'-[βγimido]triphosphate;    PLC;    phospholipase C;   

DOI  :  10.1016/0014-5793(90)80204-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have recently characterised the presence of a Ca²⁺-mobilising receptor for ATP which stimulates exocytosis in differentiated HL60 cells. Here we demonstrate that the Undifferentiated HL60 cells also respond to extracellular ATP by stimulating an increase in inositol phosphates and exocytosis. Of the nucleotides (ATP, UTP, ITP, ATPyS, AppNHp, XTP, CTP, GTP, 8-Br-ATP and GTPγS) that were active in stimulating inositol phosphate formation, only UTP, ATP, ITP, ATPγS and AppNHp were active in stimulating secretion. On differentiation, the extent of secretion due to the purinergic agonists ATP, ITP, ATPγS and AppNHp remained unchanged whilst secretion due to UTP, a pyrimidine, was substantially increased. These results indicate that the effect of ATP and UTP may be mediated via separate purinergic and pyrimidinergic receptors, respectively.

【 授权许可】

Unknown