Molecular cloning and functional expression of a novel rat heart P2X purinoceptor

FEBS Letters

Garcia-Guzman, Miguel²  Soto, Florentina²  Stühmer, Walter²  Laube, Bodo¹ 

[1] Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstr. 46, D-60528 Frankfurt, Germany;Department of Molecular Biology of Neuronal Signals, Max-Planck-Institute for Experimental Medicine, Hermann-Rein-Str. 3, D-37075 Göttingen, Germany

关键词: Purine receptor;    ATP receptor;    PPADS;    Suramin;    Heart;    CNS;    α;    β-meATP;    α;    β-methylene-ATP;    β;    γ-meATP;    β;    γ-methylene-ATP;    PPADS;    pyridoxal phosphate 6-azophenyl-2′;    4′-disulphonic acid;    TEA-Cl;    tetraethylammonium chloride;    2MeSATP;    2-methylthio-ATP;    EC50;    half-maximal effective concentration;    n H;    Hill coefficient;    N;    number of determinations or cells;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(96)00499-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we describe a novel purinergic receptor, the P2X5 receptor, cloned from rat heart. The full-length cDNA encodes a protein 455 amino acids long which shares an overall identity of 40–47% with other members of the P2X purinergic receptor family. P2X5 mRNA transcripts are found predominantly in rat heart but are also present in brain, spinal cord and adrenal gland. Functional expression of the recombinant receptor in HEK-293 cells shows a current that resembles mostly the P2X2 phenotype: the ATP-activated current reveals little agonist desensitization, is not activated by α,β-meATP and is completely blocked by suramin and PPADS.

【 授权许可】

Unknown