ATP stimulates secretion in human neutrophils and HL60 cells via a pertussis toxin‐sensitive guanine nucleotide‐binding protein coupled to phospholipase C

FEBS Letters

Stutchfield, Jane¹  Cockcroft, Shamshad¹ 

[1]Department of Experimental Pathology, School of Medicine, University College London, University Street, London WC1E 6JJ, England

关键词: ATP receptor;    Ca2+;    cytosolic;    Inositol phosphate;    fMetLeuPhe;    (HL60 cell;    Human neutrophil);    ATPγS;    adenosine 5′-[γ-thio]triphosphate;    AppNHp;    adenosine 5′-[β;    γ-imido]triphosphate;    AppCH2P;    adenosine 5′-[β;    γ-methylene]triphosphate;    G-protein;    guanine nucleotide-binding protein;   

DOI  :  10.1016/0014-5793(89)80184-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human neutrophils and HL60 cells respond to extracellular ATP by causing exocytotic secretion. Secretion is accompanied by increases in inositol phosphates and a rise in cytosol Ca²⁺. The responses to ATP are blocked by pertussis toxin pretreatment, indicating the involvement of a guanine nucleotide regulatory protein. Other nucleotides that are active in promoting secretion are ATPγS, UTP, ITP and AppNHp, whilst 8-bromo-ATP, AppCH₂p, ADP, AMP and adenosine are inactive.

【 授权许可】

Unknown