Ca2+ uptake and IP3‐induced Ca2+ release in permeabilized human lymphocytes

FEBS Letters

Eberl, G.¹  Schnell, K.¹ 

[1] Institut für Physiologie, Universität Regensburg, D-8400 Regensburg, FRG

关键词: Ca2+;    Inositol phosphate;    Lymphocyte;    (Human);   

DOI  :  10.1016/0014-5793(87)80400-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ⁴⁵Ca²⁺ uptake and ⁴⁵Ca²⁺ release in saponin-permeabilized human lymphocytes were studied. An ATP-dependent Ca²⁺ uptake into a nonmitochondrial, intracellular Ca²⁺ store is observed which is approx. 2 orders of magnitude greater than the ATP-independent Ca²⁺ uptake. The Ca²⁺ uptake is inhibited by vanadate, but it is insensitive to oligomycin and ruthenium red. IP₃ induces dose-dependent ⁴⁵Ca²⁺ release. For half-maximum Ca²⁺ release 0.25–0.5 μM IP₃ is required. The results of our studies suggest that ⁴⁵Ca²⁺ is predominantly stored within the endoplasmic reticulum of the lymphocytes.

【 授权许可】

Unknown