Muscarinic stimulation of inositol phosphate accumulation and acid secretion in gastric fundic mucosal cells

FEBS Letters

Guillon, Gilles¹  Baudière, Bruno²  Bali, Jean-Pierre²  Jard, Serge¹ 

[1] Centre CNRS-INSERM de Pharmacologie-Endocrinologie, rue de la Cardonille, 34033 Montpellier Cedex, France;ER CNRS 228, Faculté de Pharmacie, 15 av. C. Flahault, 34060 Montpellier Cedex, France

关键词: Muscarinic receptor;    Gastric acid secretion;    Ca2+;    Inositol phosphate;    (Fundic mucosal cell);    IP;    inositol phosphates;    IP1;    inositol monophosphate;    IP2;    inositol bisphosphate;    IP3;    inositol trisphosphate;    PI;    phosphatidylinositol;    PIP;    phosphatidylinositol 4-monophosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    CCh;    carbamylcholine (carbachol);    NMS;    N-methylscopolamine;    AP;    aminopyrine;   

DOI  :  10.1016/0014-5793(86)80429-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The muscarinic agonist, carbachol (CCh), was shown to stimulate the production of inositol phosphates (IP) in isolated cells from rabbit fundic mucosa. This stimulatory effect was time- and dose-dependent: EC₅₀ values for IP₁, IP₂ and IP₃ accumulation were not statistically different. The mean value was 30 ± 8 μM (n= 6). The corresponding maximal stimulation (% of basal value) observed after 20 min incubation in the presence of 100 μM CCh was 160 ± 15%. CCh-induced IP accumulation was abolished by atropine (K _i = 0.32 ± 0.18 nM (n=3)). The CCh concentrations leading to half-maximal inhibition of N-[³H]methylscopolamine binding and half-maximal IP accumulation were similar. The half-maximal value for CCh-induced aminopyrine accumulation was 8-times lower. These results indicate that IP₃-mediated mobilization of intracellular Ca²⁺ might be involved in CCh-induced acid secretion by parietal cells.

【 授权许可】

Unknown