Pertussis toxin distinguishes between muscarinic receptor‐mediated inhibition of adenylate cyclase and stimulation of phosphoinositide hydrolysis in Flow 9000 cells

FEBS Letters

Lo, William W.Y.¹  Hughes, John¹ 

[1] Parke-Davis Research Unit, Addenbrookes Hospital Site, Hills Road, Cambridge CB2 2QB, England

关键词: G-protein;    Muscarinic receptor;    Pertussis toxin;    Inositol phosphate;    cyclic AMP;    (Flow 9000 cell);    G-proteins;    guanine nucleotide-binding proteins;    Gi;    inhibitory G-protein;    [3H]IP;    total [3H]inositol phosphates;    PI;    polyphosphoinositides;    GTP[S];    guanosine 5′-O-(thiotriphosphate);   

DOI  :  10.1016/0014-5793(87)80894-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pretreatment of human embryonic pituitary tumour cells (Flow 9000) with pertussis toxin significantly reduced carbachol-mediated inhibition of isoprenaline and prostaglandin E₂ stimulation of cyclic AMP formation. This is in accord with an action on the inhibitory G_i-protein by pertussis toxin. In contrast, pertussis toxin-pretreatment had no effect on either muscarinic agonist or GTP[S] (a non-hydrolysable GTP analogue) stimulation of [³H]inositol phosphate production in intact and permeabilized [³H]inositol-prelabelled Flow 9000 cells, respectively. These results suggest that muscarinic receptors are linked to the inhibition of adenylate cyclase and the stimulation of phosphoinositidase C via two different G-proteins in Flow 9000 cells.

【 授权许可】

Unknown