Carbachol and oxytocin stimulate the generation of inositol phosphates in the guinea pig myometrium

FEBS Letters

Leiber, Denis¹  Harbon, Simone¹  Marc, Sylvie¹ 

[1] Laboratoire d'Endocrinologie et Régulations Cellulaires, CNRs VA 1131, Bt 432, Université Paris Sud, 91405 Orsay Cedex, France

关键词: (Myometrium);    Muscarinic receptor;    Oxytocin receptor;    Inositol phosphate;    Phospholipase C;    Contraction;    PIP2;    phosphatidylinositol 4;    5-biphosphate;    IP1;    inositol monophosphate;    IP2;    inositol biphosphate;    IP3;    inositol triphosphate;    d(CH2)5-D-Phe2;    Ile4AVP;    [1-β-(mercapto-β;    β-cyclopentamethylenepropionic acid);    2-D-phenylalanine;    4-isoleucine]arginine-vasopressin;   

DOI  :  10.1016/0014-5793(86)80561-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the guinea pig myometrium prelabelled with myo-[2-³H]inositol, carbachol and oxytocin enhanced a concentration-dependent and rapid release of IP₃ which preceded that of IP₂ and IP₁. The specific receptor-mediated phospholipase C activation degrading PIP₂ to IP₃ did not require the presence of extracellular Ca²⁺. The ionophore A23187 as well as K⁺ depolarization failed to increase inositol phosphate accumulation. It is proposed that IP₃ could have a role in the contraction of uterine smooth muscle elicited by the activation of muscarinic as well as of oxytocin receptors.

【 授权许可】

Unknown