Mastoparan inhibits phosphoinositide hydrolysis via pertussis toxin‐intensive G‐protein in human astrocytoma cells

FEBS Letters

Nakahata, Norimichi¹  Matsuoka, Isao¹  Nakanishi, Hironori¹  Abe, Marilene Tamiko¹ 

[1] Department of Pharmacology, Fukushima Medical College, Hikarigaoka 1, Fukuishima 960-12, Japan

关键词: Mastoparan;    Phospholipase C;    GTP binding protein;    Pertussis toxin;    Inositol phosphate;    IAP;    islet activating protein (pertussis toxin);    GTPγS;    guanosine 5'-[γ-thio]triphosphate;    G-protein;    guanine nucleotide regulatory protein;    cyclic AMP;    adenosine 3';    5'-mono-phosphate;    DMEM;    Dulbecco's modified Eagle's medium;    EMEM;    Eagle's minimum essential medium;   

DOI  :  10.1016/0014-5793(90)80074-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mastoparan inhibited [³H]inositol phosphate accumulation induced by carbachol as well as cyclic AMP accumulation induced by isoproterenol in 1321N1 human astrocytoma cells. Mastoparan inhibited GTPγS-induced, but not Ca²⁺-induced, [³H]inositol phosphate accumulation in membrane preparations with an IC ₅₀ of approximately 10 μM. The inhibitory effect of mastoparan on carbachol-induced [³pH]inositol phosphate accumulation was resistant to pertussis toxin (IAP) treatment in intact cells. These results suggest that mastoparan inhibits phospholipase C in human astrocytoma cells via a GTP binding protein, which is not a substrate for IAP.

【 授权许可】

Unknown