Pertussis toxin inhibits chemotactic factor‐induced phospholipase C stimulation and lysosomal enzyme secretion in rabbit neutrophils

FEBS Letters

Rubin, Ronald P.¹  Bradford, Peter G.¹ 

[1] Division of Cellular Pharmacology, Medical College of Virginia, Richmond, VA 23298-0001, USA

关键词: Pertussis toxin;    Rabbit neutrophil;    Phospholipase C;    Inositol trisphosphate;    Lysosomal enzyme secretion;    PtdIns(4;    5)P2;    phosphatidylinositol 4;    5-bisphosphate;    InsP3;    inositol trisphosphate;    fMet-Leu-Phe;    formylmethionylleucyl-phenylalanine;    LTB4;    leukotriene B4;    PT;    pertussis toxin;    NAGA;    N-acetylglucosaminidase;    DMSO;    dimethyl sulphoxide;   

DOI  :  10.1016/0014-5793(85)80801-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pertussis toxin suppressed [³²P]polyphosphoinositide breakdown and lysosomal enzyme secretion induced by fMet-Leu-Phe in rabbit neutrophils. Likewise, fMet-Leu-Phe- or leukotriene B4-evoked (³H]inositol trisphosphate accumulation was inhibited by the toxin. These findings, taken together with evidence that pertussis toxin specifically causes inactivation of the guanine nucleotide binding protein (N_i), suggests that guanine nucleotide binding proteins may mediate coupling between calcium-mobilising receptors and phospholipase C-mediated reactions in rabbit neutrophils.

【 授权许可】

Unknown