δ Opioid receptor mediates phospholipase C activation via Gi in Xenopus oocytes

FEBS Letters

Fukushima, Nobuyuki¹  Ueda, Hiroshi¹  Misu, Yoshimi¹  Miyamae, Takeaki¹ 

[1] Department of Pharmacology, Yokohama City University School of Medicine, Yokohama 236, Japan

关键词: δ-Opioid receptor;    Pertussis toxin;    GTP-binding protein;    Signal transduction;    Phospholipase C;    Xenopus oocyte;   

DOI  :  10.1016/0014-5793(93)80677-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cloned mouse δ-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid δ-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the α subunit of G_i1. The δ-agonist-induced current was blocked by naltrindol, a δ-specific antagonist. The δ-agonist evoked no or very weak currents in oocytes with the α subunit of G_q or G_o. These findings indicate the functional coupling between the opioid δ-receptor and phospholipase C through an activation of G_i.

【 授权许可】

Unknown