Probable occurrence of toxin‐susceptible G proteins in the nematode Caenorhabditis elegans

FEBS Letters

Ui, Michio¹  Tada, Shusuke¹  Arata, Yoichiro¹ 

[1] Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Tokyo 113, Japan

关键词: GTP-binding protein;    Pertussis toxin;    Islet-activating protein;    Cholera toxin;    Adenylate cyclase;    Caenorhabditis elegans;   

DOI  :  10.1016/0014-5793(92)80167-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pertussis toxins, islet-activating protein (IAP), and cholera toxin ADP-ribosylated 40 kDa and 45 kDa proteins in membrane preparations from Caenorhabditis elegans. Proteins with the same molecular weights were recognized in the same membranes by an antibody that had been raised against a peptic common to α-subunits of mammalian αβγ-heterotrimeric G proteins. The antibody produced immunoprecipitation with the 40 kDa protein ³²P-labeled by IAP, A 35 kDa protein immunochemically indistinguishable from β-component of mammalian G proteins was also found in C. elegans membranes. The membranes displayed adenylate cyclase activity which was highly sensitive to forskolin and GPT analogues, whose action was antagonized by GDPβS. Receptor-coupled regulation of adenylate cyclase thus appears to be mediated by mammalian-type G proteins in C. elegans as well.

【 授权许可】

Unknown