Fluoride is a strong and specific inhibitor of (asymmetrical) Ap4A hydrolases

FEBS Letters

Guranowski, Andrzej¹ 

[1] Katedra Biochemii, Akademia Rolnicza, ul. Wolyńska 35, PL-60-637 Poznań, Poland

关键词: Fluoride;    Enzyme inhibition;    Ap4A hydrolase;    Ap4A;    P 1;    P 4-bis(5'-adenosyl)tetraphosphate;    Ap3A;    P 1;    P 3-bis(5'-adenosyl)triphosphate;    Ap4G;    adenosine 5'-P 1-tetraphospho-P 4-5'''-guanosine;    Ap5A;    P 1;    P 5-bis(5-'adenosyl)pentaphosphate;   

DOI  :  10.1016/0014-5793(90)80190-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fluoride acts as a noncompetitive, strong inhibitor of (asymmetrical) Ap₄A hydrolases (EC 3.6.1.17). The K _i values estimated for the enzymes isolated from seeds of some higher plants (yellow lupin, sunflower and marrow) are in the range of 2–3 μM and I ₅₀ for the hydrolase from a mammalian tissue (beef liver) is 20 μM. The anion, up to 25 mM, does not affect the following other enzymes which are able to degrade the bis(5'-nucleosidyl)-oligophosphates: Escherichia coli (symmetrical) Ap₄A hydrolase (EC 3.6.1.41), yeast Ap₄A phosphorylase (EC 2.7.7.53), yellow lupin Ap₃A hydrolase (EC 3.6.1.29) and phosphodiesterase (EC 3.1.4.1). None of halogenic anions but fluoride affects the activity of (asymmetrical) Ap₄A hydrolases. Usefulness of the fluoride effect for the in vivo studies on the Ap₄A metabolism is shortly discussed.

【 授权许可】

Unknown