T4 DNA ligase synthesizes dinucleoside polyphosphates

FEBS Letters

Madrid, Olga¹  Martín, Daniel¹  Günther Sillero, María A.¹  Sillero, Antonio¹  Atencia, Eva Ana¹ 

[1] Departamento de Bioquímica, Instituto de Investigaciones Biomédicas, CSIC, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 29029 Madrid, Spain

关键词: Ap3A;    Ap4A;    p4A;    FHIT gene;    T4 DNA ligase;    Dinucleoside polyphosphate;    Ap3A;    diadenosine triphosphate or adenosine(5′)-triphospho(5′)adenosine;    Ap4A;    diadenosine tetraphosphate or adenosine(5′)tetraphospho(5′)adenosine;    Ap4dA;    adenosine(5′)tetraphospho(5′)2′-deoxyadenosine;    Ap4G;    adenosine(5′)tetraphospho(5′)guanosine;    Ap4N;    adenosine(5′)tetraphospho(5′)nucleoside;    NpnN;    dinucleoside polyphosphates;    meta P3;    trimetaphosphate;    P3;    tripolyphosphate;    p4A;    adenosine 5′-tetraphosphate;   

DOI  :  10.1016/S0014-5793(98)00932-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

T4 DNA ligase (EC 6.5.1.1), one of the most widely used enzymes in genetic engineering, transfers AMP from the E-AMP complex to tripolyphosphate, ADP, ATP, GTP or dATP producing p₄A, Ap₃A, Ap₄A, Ap₄G and Ap₄dA, respectively. Nicked DNA competes very effectively with GTP for the synthesis of Ap₄G and, conversely, tripolyphosphate (or GTP) inhibits the ligation of DNA by the ligase. As T4 DNA ligase has similar requirements for ATP as the mammalian DNA ligase(s), the latter enzyme(s) could also synthesize dinucleoside polyphosphates. The present report may be related to the recent finding that human Fhit (fragile histidine triad) protein, encoded by the FHIT putative tumor suppressor gene, is a typical dinucleoside 5′,5″-P¹,P³-triphosphate (Ap₃A) hydrolase (EC 3.6.1.29).

【 授权许可】

Unknown