p1,p4‐Diadenosine 5′‐tetraphosphate induces the uptake of arginine and citrulline by a pore on the plasma membrane of bovine aortic endothelial cells

FEBS Letters

Rosenberg, Christina R.²  Hilderman, Richard H.¹  Cooper, Heidi M.² 

[1] Departments of Microbiology/Molecular Medicine, Biological Sciences, Vascular Research Laboratory and the South Carolina Experiment Station, Clemson University, Clemson, SC 29634-1909, USA;Department of Biological Sciences, Clemson University, Clemson, SC 29634-1903, USA

关键词: Ap4A;    Pore;    L-Arg uptake;    Bovine endothelial cell;   

DOI  :  10.1016/S0014-5793(00)02187-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously demonstrated that p¹,p⁴-diadenosine 5′-tetraphosphate (Ap₄A) induces the release of nitric oxide (NO) and modulates the uptake of extracellular L-arginine (L-Arg) and L-citrulline (L-Cit) by bovine aortic endothelial cells (BAEC) [Hilderman, R.H. and Christensen, E.F. (1998) FEBS Lett. 427, 320–324 and Hilderman, R.H., Casey, T.E. and Pojoga, L.H. (2000) Arch. Biochem. Biophys. 375, 124–130]. In this communication we report that extracellular Ap₄A enhances the uptake of L-Arg and L-Cit through a pore on the plasma membrane of BAEC that is selective for these two amino acids. We also demonstrate that Ap₂A, which induces NO release, enhances L-Arg uptake while Ap₅A, a vasoconstrictor, does not enhance the uptake of L-Arg. The potential physiological significance of the uptake of these two amino acids in relation to NO synthesis is discussed.

【 授权许可】

Unknown