Evidence for two independent pathways in the stimulation of steroidogenesis by luteinizing hormone involving chloride channels and cyclic AMP

FEBS Letters

Choi, Michael S.K.¹  A. Cooke, Brian¹ 

[1] Department of Biochemistry, Royal Free Hospital School of Medicine, University of London, Rowland Hill Street, London NW3 2PF, England

关键词: Luteinizing hormone;    Cyclic AMP;    Chloride channel;    Calcium;    Leydig cell;    Steroidogenesis;   

DOI  :  10.1016/0014-5793(90)80602-F

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible role of chloride channels in luteinizing hormone (LH) action on steroidogenesis in rat Leydig cells had been investigated. A chloride channel blocker, SITS (4-acetamido-4'-isothiocyanatostilbene-2,2'-disulphonic acid), inhibited LH-stimulated steroidogenesis at low (⩽ 1 ), but not at high (100 ) LH concentrations. In addition, dibutyryl cyclic AMP- and forskolin-stimulated steroidogenesis was unaffected by SITS. The removal of extracellular chloride potentiated steroidogenesis stimulated by submaximal but not maximal doses of LH. These results suggest that at low levels of LH, steroidogenesis depends on chloride channels whereas with high levels, cyclic AMP is the mediator of LH action.

【 授权许可】

Unknown