cAMP directly facilitates Ca‐induced exocytosis in bovine lactotrophs

FEBS Letters

Zorec, Robert¹  Mason, William T.²  Sikdar, Sujit K.² 

[1] Institute of Pathophysiology, School of Medicine, 61105 Ljubljana, Yugoslavia;Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, CB2 4AT, UK

关键词: Calcium;    Exocytosis;    Cyclic AMP;    Hormone;    Secretion;    Prolactin;    Anterior pituitary;   

DOI  :  10.1016/0014-5793(90)81072-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have used the whole cell patch clamp technique on single prolactin-secreting bovine lactotrophs to measure plasma membrane capacitance (C _m), an index of membrane surface area, under voltage-clamp during cytosol dialysis with Ca and cAMP. cAMP increased the magnitude and rate of Ca-induced exocytosis (C _m increase) without affecting membrane conductance; however, cAMP had no detectable effect on C _m when intracellular Ca was low. We thus report new evidence that cAMP can facilitate Ca-induced secretion in a synergistic fashion, by acting directly on the secretory apparatus, independently of membrane conductance activation.

【 授权许可】

Unknown