Evidence for the requirement of proteolysis in LH stimulated cyclic AMP production and steroidogenesis in Leydig cells

FEBS Letters

Phipp, Laura H.¹  West, Anthony P.¹  Cooke, Britin A.¹ 

[1] Department of Biochemistry, Royal Free Hospital School of Medicine, University of London, Rowland Hill Street, London NW3 2PF, UK

关键词: Luteinizing hormone;    Steroidogenesis;    Cyclic AMP;    Proteolysis;   

DOI  :  10.1016/0014-5793(91)80486-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have investigated the effect of protease activity on cyclic AMP production and steroidogenesis in rat testis, mouse testis and mouse tumour Leydig (MA10) cells. LH-, dibutyryl cyclic AMP-, and forskolin-stimulated steroidogenesis, but not 22R(OH) cholesterol conversion to pregnenolone, was inhibited by protease inhibitors. In mouse Leydig cells, LH but not forskolin or cholera toxin stimulated cyclic AMP production was inhibited by protease inhibitors. These results suggest that steroidogenesis in Leydig cells requires proteolysis before the conversion of cholesterol to pregnenolone. In the mouse but not rat Leydig cells, LH-stimulated cyclic AMP production is also dependent on proteolysis.

【 授权许可】

Unknown