Suramin, an anti‐cancer drug, inhibits protein kinase C and induces differentiation in neuroblastoma cell clone NB2A

FEBS Letters

Boscoboinik, Daniel¹  Azzi, Angelo¹  Hensey, Carmel E.¹ 

[1] Institut für Biochemie und Molekularbiologie, Universität Bern, CH-3012 Bern, Switzerland

关键词: Protein kinase C;    Neuroblastoma;    Suramin;    Differentiation;    Antineoplastic therapy;    Proliferation inhibition;    PKC;    protein kinase C;    FCS;    fetal calf serum;    DMEM;    Dulbecco's modified Eagle's medium;    EGTA;    [ethylene-bis(oxyethylenenitrilo)]tetra-acetic acid;    EDTA;    ethylenediamine tetra-acetic acid;    DTT;    dithiothreitol;   

DOI  :  10.1016/0014-5793(89)81639-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein kinase C purified from rat brain was found to be inhibited by suramin, a substance used originally in the therapy of antitrypanosomic infections and more recently proposed as antineoplastic agent. The inhibition of suramin was competitive with one of the substrates of the enzyme, ATP with a K _i of 10 μM. At concentrations adequate to inhibit the isolated enzyme, suramin was shown to slow the rate of proliferation of neuroblastoma NB2A cells in vitro and to induce their differentiation as evidenced by typical morphological changes.

【 授权许可】

Unknown