Down‐regulation of protein kinase Cα and γ and enhanced TPA‐induced neurite formation in DAN‐transfected neuroblastoma cells

FEBS Letters

Nakagawara, Akira²  Kondo, Kazuhiro²  Nakamura, Yohko²  Ozaki, Toshinori²  Saido, Takaomi¹  Sakiyama, Shigeru²  Hiwasa, Takaki³ 

[1] Laboratory for Proteolytic Neuroscience, RIKEN Brain Science Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0106, Japan;Division of Biochemistry, Chiba Cancer Center Research Institute, 666-2, Nitona-cho, Chuo-ku, Chiba 260-0801, Japan;Department of Biochemistry, School of Medicine, Chiba University, Inohana 1-8-1, Chuo-ku, Chiba 260-8670, Japan

关键词: DAN;    Tumor suppressor;    Protein kinase C;    Calpain;    Neuroblastoma;    BMP;    bone morphogenetic protein;    Dex;    dexamethasone;    DMSO;    dimethylsulfoxide;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol 13-acetate;    ZLLH;    carbobenzoxy-leucyl-leucinal;   

DOI  :  10.1016/S0014-5793(98)01412-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DAN gene was first isolated by differential screening between rat 3Y1 and v-src-transformed 3Y1 cells and showed a tumor-suppressive activity toward v-src-transformed 3Y1 cells. When DAN-transfected neuroblastoma cells were treated with a tumor promoter phorbol ester, TPA, neurite-like processes appeared within 2 h whereas no apparent change was observed in the parent and vector-transfected cells up to 8 h. This suggests some difference in TPA-receptor, protein kinase C (PKC), between DAN-transfectants and the control cells. DAN-transfected SH-SY5Y cells showed complete loss in PKCα and a large decrease in PKCγ. Similar down-regulation in PKCα and PKCγ was also observed in DAN-transfected Ha-ras-transformed NIH 3T3 cells. The decreased level of PKCα was partially recovered after treatment with a calpain inhibitor, ZLLH. A 150-kDa proteolytic product of a calpain-specific substrate, non-erythroid α-spectrin, was detectable in DAN-transfected SH-SY5Y cells but not in the parent or vector-transfected control cells. This suggests that DAN-transfected cells contain activated calpain which may cause down-regulation of PKC and hence induce the altered TPA response.

【 授权许可】

Unknown