Nucleotide sequence of the maltotetraohydrolase gene from Pseudomonas saccharophila

FEBS Letters

Takano, Toshiya²  Zhou, Jianhua¹  Baba, Tadashi¹  Arai, Yuji¹  Kobayashi, Shoichi² 

[1] Institute of Applied Biochemistry, University of Tsukuba and *National Food Research Institute, Tsukuba City, Ibaraki 305, Japan;National Food Research Institute, Tsukuba City, Ibaraki 305, Japan

关键词: Maltotetraohydrolase;    Gene cloning;    Nucleotide sequence;    Amino acid sequence;    (Pseudomonas saccharophila);   

DOI  :  10.1016/0014-5793(89)81056-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of the Pseudomonas saccharophila gene encoding maltotetraohydrolase (G₄-forming amylase) has been determined. The coding region for the G₄-forming amylase precursor contained 1653 nucleotides. The deduced precursor protein included an N-terminal 21-residue putative signal peptide; the deduced mature form of G₄-forming amylase contains 530 amino acid residues with a calculated molecular mass of 57 740 Da. Sequence similarities between the G₄-forming amylase and other amylolytic enzymes of species ranging from prokaryotes to eukaryotes are quite limited. However, three regions, which are involved in both the catalytic and substrate-binding sites of various amylolytic enzymes, are highly conserved in the G₄-forming amylase of P. saccharophila.

【 授权许可】

Unknown