Yeast vacuolar aminopeptidase yscI

FEBS Letters

García-Alvarez, Nieves¹  Suárez-Rendueles, Paz¹  Cueva, Rosario¹ 

[1] Departamento de Biología Funcional, Area de Bioquímica y Biología Molecular, Universidad de Oviedo, E-33071 Oviedo, Spain

关键词: Aminopeptidase;    Peptidase;    vacuolar;    Gene cloning;    Nucleotide sequence;    (Saccharomyces cerevisiae;    Yeast);   

DOI  :  10.1016/0014-5793(89)81510-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The structural gene, APE1, (LAP4), for the vacuolar aminopeptidase I of Saccharomyces cerevisiae was cloned with the aid of a staining technique which permitted monitoring of aminopeptidase activity in yeast colonies. Genetic linkage data demonstrate that integrated copies of the cloned gene map to the APEI locus. The nucleotide sequence of the cloned gene was determined. The open reading frame of APEI consists of 514 codons and, therefore, encodes a larger protein (MW 57 003) than the reported mature aminopeptidase yscI (MW 44 800), suggesting that proteolytic processing must occur. A 1.75-kb mRNA, which is made in substantial amounts only when yeast cells have exhausted the glucose supply, was identified.

【 授权许可】

Unknown