期刊论文详细信息
FEBS Letters | |
Is phospholipid a required cofactor for the activity of mammalian signal peptidase? | |
Uchida, Masahide1  Watanabe, Yasuhiro1  Fujimoto, Yukio1  | |
[1] Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Katsuraoka-cho, Otaru, Hokkaido 047-02, Japan | |
关键词: Peptidase; Phospholipid; Delipidation; Detergent; NP-40; Nonidet P-40; PMSF; phenylmethylsulfonyl fluoride; Mes; 2-(N-morpholino)ethane-sulfonic acid; pre-hPL; pre-human placental lactogen; SBPC; soybean phosphatidylcholine; | |
DOI : 10.1016/0014-5793(86)81165-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
To determine whether phospholipid is required for the activity of mammalian signal peptidase, the enzyme was partially purified from porcine pancreas and then extensively freed of phospholipid by SP-Sephadex C-50 chromatography. The delipidated enzyme showed signal peptidase activity, with a low concentration of detergent. Phospholipid was found to release the enzyme from the inhibition due to excess detergent.
【 授权许可】
Unknown
【 预 览 】
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