Is phospholipid a required cofactor for the activity of mammalian signal peptidase?

FEBS Letters

Uchida, Masahide¹  Watanabe, Yasuhiro¹  Fujimoto, Yukio¹ 

[1] Department of Clinical Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Katsuraoka-cho, Otaru, Hokkaido 047-02, Japan

关键词: Peptidase;    Phospholipid;    Delipidation;    Detergent;    NP-40;    Nonidet P-40;    PMSF;    phenylmethylsulfonyl fluoride;    Mes;    2-(N-morpholino)ethane-sulfonic acid;    pre-hPL;    pre-human placental lactogen;    SBPC;    soybean phosphatidylcholine;   

DOI  :  10.1016/0014-5793(86)81165-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To determine whether phospholipid is required for the activity of mammalian signal peptidase, the enzyme was partially purified from porcine pancreas and then extensively freed of phospholipid by SP-Sephadex C-50 chromatography. The delipidated enzyme showed signal peptidase activity, with a low concentration of detergent. Phospholipid was found to release the enzyme from the inhibition due to excess detergent.

【 授权许可】

Unknown