Cloning and sequence analysis of the phenylalanyl‐tRNA synthetase genes (pheST) from Thermus thermophilus

FEBS Letters

Hennecke, Hauke¹  Keller, Barbara¹  Kast, Peter¹ 

[1] Mikrobiologisches Institut, Eidgenössische Technische Hochschule, Schmelzbergstrasse 7, CH-8092 Zürich, Switzerland

关键词: Class II aminoacyl-tRNA synthetase;    Gene cloning;    Nucleotide sequence;    Phenylalanyl-tRNA synthetase;    pheS and pheT gene;    bp;    basepair(s);    kb;    kilobasepair(s);    PheRS;    phenylalanyl-tRNA synthetase;   

DOI  :  10.1016/0014-5793(92)80215-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

While crystals suitable for X-ray diffraction analyses are available of phenylalanyl-tRNA synthetase (PheRS) from the thermophilic bacterium Thermus thermophilus, neither the primary structure of its constituent α and β subunits nor the nucleotide sequence of the corresponding pheS and pheT genes were known. Using specific oligonucleotides of conserved pheS regions that were adapted to the T. thermophilus codon usage, we identified, cloned and subsequently sequenced the pheST genes of this bacterium. The sequences reported here will greatly aid in the three-dimensional structure determination of T. thermophilus PheRS, a heterotetrameric (α,β₂) class II aminoacyl-tRNA synthetase.

【 授权许可】

Unknown