Neutralizing monoclonal antibody specific for α‐bungarotoxin: Preparation and characterization of the antibody, and localization of antigenic region of α‐bungarotoxin

FEBS Letters

Kase, Ryoichi¹  Inagaki, Fuyuhiko¹  Hayashi, Kyozo³  Tanoue, Kenjiro²  Kitagawa, Hisayo² 

[1] Department of Molecular Physiology, The Tokyo Metropolitan Institute of Medical Science, 18-22, Honkomagome 3-chome, Bunkyo-ku, Tokyo 113, Japan;Department of Cardiovascular Research, The Tokyo Metropolitan Institute of Medical Science, 18-22, Honkomagome 3-chome, Bunkyo-ku, Tokyo 113, Japan;Department of Pharmaceutics, Gifu Pharmaceutical University, Gifu 502, Japan

关键词: Monoclonal antibody;    Bungarotoxin;    α-;    Antigenic determinant;    αBTx;    α-bungarotoxin;    Tx B;    toxin B;    Tx III;    toxin III;    Ls III;    Laticauda semifasciata III;    MaB;    monoclonal antibody;   

DOI  :  10.1016/0014-5793(89)81018-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We prepared an α-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of α-bungarotoxin. The antigenic determinant was located on the peptide fragment containing S34-S35-R36-G37-K38, which forms a part of the expected site that binds to the acetylcholine receptor proteins

【 授权许可】

Unknown