Localization of a sequential B‐epitope in the VP2 protein of hepatitis A virus

FEBS Letters

Kulik, Liudmila N.¹  Ostrovsky, Andrey G.²  Tchikin, Leonid D.²  Ivanov, Vadim S.²  Ivanov, Vadim T.² 

[1] Institute of Bioorganic Chemistry, Byelorussian Academy of Sciences, Minsk, Byelorussia;Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117871 Moscow, GSP-7, Russian Federation

关键词: Synthetic peptide;    Antigenic determinant;    HAV binding antibody;    Antipeptide antibody;    Monoclonal antibody;    HAV;    hepatitis A virus;    MAP;    multiple antigen peptide;    mAb;    monoclonal antibody;    KLH;    keyhole limpet haemocyanin;    Ova;    ovalbumin;    BSA;    bovine serum albumin;    ELISA;    enzyme-linked immunosorbent assay;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(95)00520-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A set of synthetic peptides derived from the capsid proteins of hepatitis A virus was used to search for B-epitopes. Peptides from the 115–139 region of the VP1 protein, from the 69–99 region of the VP2 protein and peptide 137–150 from the VP3 protein were found to react with monoclonal and polyclonal anti-HAV antibodies. MAPs based on 64–80 and 66–80 fragments of VP3 were reactive as well. Peptides, their conjugates with protein carriers and MAPs were used for antipeptide antibody production. Only free peptide 69–99 from the VP2 protein caused formation of HAV binding antibodies.

【 授权许可】

Unknown