Prediction of sequential antigenic regions in proteins

FEBS Letters

Weijer, Wicher J.¹  Welling, Gjalt W.¹  van der Zee, Ruurd¹  Welling-Wester, Sytske¹ 

[1] Laboratorium voor Medische Microbiologie. Rijksuniversiteit Groningen, Oostersingel 59, 9713 EZ Groningen, The Netherlands

关键词: Antigenicity;    Prediction;    Antipeptide antibody;    Synthetic peptide;   

DOI  :  10.1016/0014-5793(85)80374-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prediction of antigenic regions in a protein will be helpful for a rational approach to the synthesis of peptides which may elicit antibodies reactive with the intact protein. Earlier methods are based on the assumption that antigenic regions are primarily hydrophilic regions at the surface of the protein molecule. The method presented here is based on the amino acid composition of known antigenic regions in 20 proteins which is compared with that of 314 proteins [(1978) Atlas of Protein Sequence and Structure, vol. 5, suppl. 3, 363-373]. Antigenicity values were derived from the differences between the two data sets. The method was applied to bovine ribonuclease, the B-subunit of cholera toxin and herpes simplex virus type 1 glycoprotein D. There was a good correlation between the predicted regions and previously determined antigenic regions.

【 授权许可】

Unknown