Functional groups on ‘Z’ DNA recognized by monoclonal antibodies

FEBS Letters

Lee, Jeremy S.¹  Latimer, Laura J.P.¹  Woodsworth, Mary L.¹ 

[1] Department of Biochemistry, University of Saskatchewan, Saskatoon, Saskatchewan S7N 0W0, Canada

关键词: Z-DNA;    8-Bromoguanine;    5-Methylcytosine;    Antigenic determinant;    Monoclonal antibody;   

DOI  :  10.1016/0014-5793(84)80267-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Both brominated poly[d(GC)] and poly[d(Gm⁵C)] form stable left-handed Z-DNA structures at physiological ionic strengths. These two antigens were use to prepare monoclonal antibodies from immunized mice. The specificity of the antibodies was studied in detail with a solid-phase radioimmune assay as well as by means of competition experiments. Both immunogens produced several relatively non-specific antibodies but two types of very specific antibody were also distinguished. The first binds poly[d(Gm⁵C)] but not brominated poly[d(GC)] while the other has the opposite specificity and will only bind the brominated polymer.

【 授权许可】

Unknown