Recombinant core particles of hepatitis B virus exposing foreign antigenic determinants on their surface

FEBS Letters

Ose, V.²  Siakkou, H.¹  Ulrich, R.¹  Gren, E.J.⁴  Loseva, V.³  Pumpen, P.⁴  Pushko, P.M.⁴  Borisova, G.P.⁴  Tsibinogin, V.V.³  Berzins, I.⁴  Rosenthal, H.A.¹ 

[1] Institut für medizinische Virologie, Bereich Medizin der Humboldt-Universität (Charité), Schumannstr., 20/21, 1040 Berlin, GDR;A. Kirhenstein's Institute of Microbiology, Latvian Academy of Sciences, Kleisti, Riga, Latvian SSR, USSR;Experimental Plant Institute of Organic Synthesis, Krustpils Str., 53, Riga, USSR;Department of Molecular Biology, Institute of Organic Synthesis, Latvian Academy of Sciences, Aizkraukles Str., 21, Riga, USSR

关键词: Hepatitis B core antigen;    PreS region;    Human immunodeficiency virus-1 transmembrane protein gp41;    Bovine leukemia virus envelope protein gp51;    Antigenic determinant;   

DOI  :  10.1016/0014-5793(89)81509-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insertion of foreign oligopeptide sequences (40–50 amino acids in length) into the Pro₁₄₄ position of hepatitis B core antigen (HBcAg) leads to the formation of chimeric capsids in Escherichia coli cells. These capsids are morphologically and immunogically similar to native HBcAg, but expose the inserted oligopeptides on their outer surface and exhibit antigenic and immunogenic characteristics of the latter. As a source of model antigenic determinants, the appropriate DNA copies excised from cloned viral genes such as the pre-S region of hepatitis B virus, the transmembrane protein gp41 of human immunodeficiency virus 1 and the envelope protein gp51 of bovine leukemia virus have been used. The localization of the inserted antigenic determinants on the surface of chimeric capsids does not depend on the presence or absence of the arginine-rich, 39 amino acid-long C terminus of HBcAg.

【 授权许可】

Unknown