Targeting of a foreign protein into the thylakoid lumen of pea chloroplasts

FEBS Letters

Robinson, Colin¹  Hulford, Andrew¹  Shackleton, Jamie B.¹  Meadows, Julie W.¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England

关键词: Protein transport;    Retargeting;    Processing;    Chloroplast;    Thylakoid lumen;   

DOI  :  10.1016/0014-5793(89)80968-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A chimaeric gene was constructed which encodes the pre-sequence of the 33 kDa protein of the oxygen-evolving complex, a thylakoid lumen protein, linked to dihydrofolate reductase, a cytoplasmic protein. The encoded fusion protein is transported into the thylakoid lumen of isolated pea chloroplasts, with concomitant removal of the pre-sequence.

【 授权许可】

Unknown