Targeting of ricin A chain into pea chloroplasts

FEBS Letters

Robinson, Colin¹  Meadows, Julie W.¹  Roberts, Lynne M.¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry, CV4 7AL, UK

关键词: Protein transport;    Retargeting;    Ricin. Chloroplast;   

DOI  :  10.1016/0014-5793(91)81169-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Achimaeric gene was constructed encoding the pre-sequence of the 33 kDa oxygen-evolving complex protein from wheat (a thylakoid lumen protein) linked to ricin A chain. The fusion protein is efficiently imported by isolated pea chloroplasts and localised partly in the stroma, with the remainder bound to the stromal surface of the thylakoids. The imported protein is fully processed by both the stromal and thylakoidal processing peptidases, indicating that partial or complete translocation across the thylakoid membrane has taken place.

【 授权许可】

Unknown