Direct evidence that the kinase activity of protein kinase C is involved in transcriptional activation through a TPA‐responsive element

FEBS Letters

Konno, Yasuhiko¹  Hata, Akiko¹  Ohno, Shigeo¹  Akita, Yoshiko¹  Suzuki, Koichi¹ 

[1] Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science, Honkomagome, Bunkyo-ku, Tokyo 113, Japan

关键词: Phorbol ester;    Tumor-promoter-responsive element;    Protein kinase C;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoyl phorbol 13-acetate;    TRE;    TPA-responsive element;    CAT;    chloramphenicol transferase;    FCS;    fetal calf serum;    IFN;    interferon;   

DOI  :  10.1016/0014-5793(89)80907-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In order to examine the involvement of protein kinase C (PKC) in the transcriptional activation of genes by TPA (12-O-tetradecanoyl phorbol 13-acetate) we have constructed a series of PKC expression plasmids. Transient expression of an active fragment of PKC in rat fibroblasts resulted in the transcriptional activation of a TRE (TPA-responsive element)-CAT chimeric gene which contains various repetitions of collagenase TREs. These provide the first direct evidence that kinase activity of PKC is involved in TPA-induced transcriptional activation through TRE.

【 授权许可】

Unknown