The stimulation of phosphorylation of intracellular proteins in GH3 rat pituitary tumour cells by phorbol esters of distinct biological activity

FEBS Letters

Evans, F.J.¹  Brooks, S.F.²  Aitken, A.² 

[1] Dept of Pharmacognosy, School of Pharmacy, University of London, 29-39 Brunswick Sq., London, England;Laboratory of Protein Structure, The National Institute for Medical Research, Mill Hill, London England

关键词: Protein kinase C;    Phorbol ester;    Protein phosphorylation;    DOPPA;    12-deoxyphorbol-13-phenyl-acetate-20-acetate;    PKC;    protein kinase C;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    SAPA;    sapintoxin A;    IP3;    inositol 1;    4;    5-trisphosphate;    DAG;    1;    2-diacylglycerol;    α-SAP Ac;    α-sapinine acetate;   

DOI  :  10.1016/0014-5793(87)80431-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using a pituitary tumour cell line (GH₃), we have studied the phosphorylation of intracellular proteins induced by phorbol esters of diverse biological activity. All the active phorbol esters, including the weakly tumour-promoting but non-platelet aggregatory compound DOPPA, stimulated the phosphorylation of a cytosolic 80 kDa protein. A protein of this molecular mass has been suggested to be a marker of PKC activity. In contrast, only TPA and the non-tumour promoting but highly active phorbol ester SAP A stimulated the phosphorylation of a 130 kDa membrane protein. The results suggest that these phorbol esters activate PKC, but induce the differential phosphorylation of a variety of intracellular proteins.

【 授权许可】

Unknown