Synthesis of human endothelin‐1 precursors in Escherichia coli

FEBS Letters

Itoh, Yasuaki¹  Onda, Haruo¹  Kimura, Chiharu¹  Ogi, Kazuhiro¹  Suzuki, Nobuhiro¹  Watanabe, Takuya¹ 

[1] Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd, Wadai- 7, Tsukuba, Ibaraki 300-42, Japan

关键词: Preproendothelin;    Endothelin precursor;    Vasoconstrictor peptide;    Gene expression system;    (E. coli);   

DOI  :  10.1016/0014-5793(89)81466-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Endothelin, the most potent vasoconstrictor found in nature, is thought to be important in the regulation of blood pressure and/or local blood distribution. Human placenta cDNA fragment encoding preproendothelin-1 (preproET- 1) and its carboxyl terminal mature precursor (C-matured precursor) was expressed in E. coli. These products were characterized by both enzyme immunoassay and Western blot analysis.

【 授权许可】

Unknown